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H9395
Sigma
α-Hemolysin from Staphylococcus aureus
lyophilized powder, Protein ~60 % by Lowry, ≥10,000 units/mg protein
| Synonym: | α-Toxin |
| CAS Number: | 94716-94-6 |
| MDL number: | MFCD00130489 |
Description
| Packaging | Package size based on protein content |
| Unit Definition | One hemolytic unit will cause 50% lysis of a 1% suspension of rabbit red blood cells in phosphate buffered saline, pH 7.0, containing 1% bovine serum albumin after 30 min at 37 °C followed by refrigeration for 30 min at 4 °C. |
| Biochem/physiol Actions | α-Hemolysin is a 33 kDa extracellular protein secreted by most strains of pathogenic Staphylococcus aureus. It is selectively hemolytic and has a marked preference for rabbit red blood cells. It induces dermonecrosis, spastic muscle paralysis, and it is lethal for laboratory animals. The toxin must be in the monomeric form to initially bind to a membrane and specific receptors are not required for binding. Upon binding to biological membranes and/or artificial membranes, self-oligomerization occurs, resulting in ring structures (hexameric aggregates) believed to represent transmembrane pores, which are permeable to ions and small metabolites. It is thought that α-hemolysin stimulates cellular phospholipases and induces a Ca2+ influx that can result in membrane disruption, leakage of cytoplasmic components, impaired membrane permeability, and osmotic lysis of the cells. |
Properties
| form | lyophilized powder |
| contains | sodium citrate buffer as balance |
| composition | Protein, ~60% Lowry |
| storage temp. | 2-8°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | 3 |
